A simplified protocol for the automated production of succinimidyl 4-[ 18F]fluorobenzoate on an IBA Synthera module

Abstract

The important peptide labelling reagent succinimidyl 4-[ 18F]fluorobenzoate ([18F]SFB) has been synthesised in 75-85% decay corrected radiochemical yield using the IBA Synthera platform (IBA Cyclotron Solutions, Louvain-la-neuve, Belgium) with the fluorodeoxyglucose- integrated fluidic processor nucleophilic and only four reagent vials in a single reactor. (4-ethoxycarbonylphenyl) trimethylammonium triflate was used as the labelling precursor and 1 M aqueous tetramethylammonium hydroxide for the hydrolysis of the intermediate ethyl 4-[18F]fluorobenzoate. N,N,N′,N′-tetramethyl-O-(N-succinimidyl)uronium tetrafluoroborate (TSTU) was then used to form [18F]SFB from 4-[18F] fluorobenzoate. By omi..